生物素-dPEG ® β-羟胺。盐酸-1000 mg

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Biotin-dPEG®3-oxyamine·HCl, product number QBD-11100, consists of a short (14 atoms, 16.6 Å) single molecular weight, discrete PEG (dPEG®) spacer functionalized at each end with biotin and an oxyamine group (as the HCl salt), respctively. This PEG biotin compound reacts with aldehydes and ketones to forms stable oxime bonds. Using this biotinylation reagent permits biotin labeling of the carbohydrate coats of glycoproteins after the introduction of aldehydes and ketones on the carbohydrate coat using enzymatic (for example, galactose oxidase) or mild chemical reaction conditions such as sodium periodate. Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or surfaces such as glass or gold. Bioconjugation research and product development and numerous clinical assays use biotinylated molecules. The amphiphilic dPEG® linker of Biotin-dPEG®3-oxyamine·HCl imparts excellent water solubility to biotin, which is usually poorly soluble in water and causes non-specific hydrophobic interactions when conjugated to biomolecules. Some commercially available biotin-aminooxy products use either no spacer or a hydrophobic alkyl chain as a spacer between biotin and the aminooxy moiety. Such products have poor solubility in water and must be dissolved in a dry organic solvent such as dimethyl sulfoxide (DMSO) immediately before use, which can be problematic under some conditions. If used on proteins, these hydrophobic products may trigger aggregation and precipitation of the biotinylated molecule. Even if aggregation and precipitation do not occur, the hydrophobic spacer can contribute to non-specific binding. Because it is amphiphilic, Biotin-dPEG®3-oxyamine·HCl can be dissolved in water or aqueous buffer and used directly in a conjugation reaction. QBD-11100 will not cause aggregation or precipitation of the biotinylated molecule. Moreover, the hydrophilicity of the dPEG® linker reduces or eliminates non-specific binding. An aminooxy group (also known as an alkoxyamine) consists of a primary terminal amine adjacent to oxygen (i.e., —ONH2). This group reacts selectively and efficiently with aldehydes to form an aldoxime linkage and with ketones to form a ketoxime linkage. An oxime bond is more stable than a hydrazone bond and does not need to be reduced to a secondary amine to stabilize it. Aldehydes and ketones occur relatively rarely in nature; however, using sodium periodate, aldehydes can be formed in glycoproteins whose carbohydrate coats contain reducing sugars. Moreover, scientific literature examples demonstrate the introduction of ketones into bacterial cell walls through liposomal fusion.
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品牌介绍

Click Chemistry Tools（CCT）公司位于美国亚利桑那州斯科茨代尔（Scottsdale,AZ）。自公司成立以来，一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外，CCT还可以提供个性化的定制产品，已满足不同用户的需求。目前CCT的产品线主要包括：Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。 Fluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，自成立以来，一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外，Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。 Quanta Biodesign公司位于美国中部的俄亥俄州，成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合，生产出他们独有的专利产品dPEG Building Blocks and Linkers，被广泛用于细胞治疗和分子诊断等研究领域。另外，还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。 目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中

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