Dde塔姆拉生物素炔-25 mg

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Trifunctional Click Chemistry Probes that incorporate a ligation handle, a biotin and a fluorophore have become a popular tool for for tandem labeling of proteins and subsequent detection or enrichment. However, due to the strong interaction between biotin and streptavidin harsh conditions are necessary for the elution of enriched proteins. This usually leads to contamination of the sample with non-specifically bound proteins and endogenously biotinlytated proteins, which complicates target identification. Cleavable Trifunctional Click Chemistry Probes overcome this major drawback of the streptavidin-biotin affinity purification. These probes contain a biotin moiety linked to a “clickable” group and fluorescent dye through a spacer arm containing a cleavable Dde linker. Dde moiety is stable to rigorous, denaturing wash conditions, acidic or basic conditions including generally applied buffer systems to which the biological sample may be exposed. At the same time Dde linker can be quantitatively cleaved under mild aqueous buffered conditions with 2% hydrazine. Another very important advantage of dual label probes over regular biotin probes is built-in control. Each step of enrichment process can easily followed either by UV-Vis (550 nm) or by more sensitive fluorescence spectroscopy. After elution form streptavidin beads target proteins containing TAMRA label can be easily distinguished from non-specifically bound proteins and endogenously biotinlytated proteins. This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.
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品牌介绍

Click Chemistry Tools（CCT）公司位于美国亚利桑那州斯科茨代尔（Scottsdale,AZ）。自公司成立以来，一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外，CCT还可以提供个性化的定制产品，已满足不同用户的需求。目前CCT的产品线主要包括：Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。 Fluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，自成立以来，一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外，Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。 Quanta Biodesign公司位于美国中部的俄亥俄州，成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合，生产出他们独有的专利产品dPEG Building Blocks and Linkers，被广泛用于细胞治疗和分子诊断等研究领域。另外，还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。 目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中

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