NHS-dPEG®生物素-50 mg

背景资料

NHS-dPEG®4-biotin, product number QBD-10200, features biotin coupled to a short, discrete tetraethylene glycol (dPEG®4) spacer and functionalized with an N-hydroxysuccinimidyl (NHS) ester of propionic acid. The amphiphilic dPEG® spacer imparts flexibility and water solubility to the normally hydrophobic biotin moiety, while the NHS ester permits biotin conjugation to free primary amines such as the side chain of lysine. This popular compound is useful for assays and techniques requiring the biotin-avidin interaction. NHS-dPEG®4-biotin provides superior performance to the widely used, but notoriously hydrophobic, NHS-LC-biotin. The amphiphilicity of QBD-10200 means that it can be dissolved in organic solvents and aqueous buffers with equal ease, and the flexible linker adds significant hydrophilicity to the conjugates formed with it. Notably, NHS-dPEG®4-biotin does not cause protein aggregation. This permits experiments that require high levels of biotin labeling to proceed without concerns about the loss of protein due to aggregation, which is a common problem when using NHS-LC-biotin. Dissolution of the product in organic solvent is not required for conjugation, thus providing easier reaction setup and greater reaction reproducibility. The dPEG® linker in PN10200 is slightly longer than the aminocaproic acid linker in LC-biotin; therefore, dPEG®4-biotin provides somewhat better access of the biotin to the avidin or streptavidin binding pocket compared to LC-biotin.
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品牌介绍

Click Chemistry Tools（CCT）公司位于美国亚利桑那州斯科茨代尔（Scottsdale,AZ）。自公司成立以来，一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外，CCT还可以提供个性化的定制产品，已满足不同用户的需求。目前CCT的产品线主要包括：Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。 Fluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，自成立以来，一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外，Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。 Quanta Biodesign公司位于美国中部的俄亥俄州，成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合，生产出他们独有的专利产品dPEG Building Blocks and Linkers，被广泛用于细胞治疗和分子诊断等研究领域。另外，还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。 目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中

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