产品详情
背景资料
Biotin-dPEG®3-MAL, product number 10201, is one of three products containing biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other. For QBD-10201, the linker is a dPEG®3 product. The flexible, short-chain, discrete PEG (dPEG®) linker imparts water solubility to conjugates formed with it. The maleimide moiety is a popular reactive group for conjugating to free thiol groups in biomolecules and other sulfhydryl compounds. Maleimide groups can be conjugated to free thiol groups in the pH range 6.5 – 7.5, forming a stable thioether linkage.
This product has proven helpful in various applications that utilize the high streptavidin-biotin binding affinity. Such applications include
FRET-based assays
probes of protein topology
protein labeling
the creation of biotinylated, dPEG®-modified nucleosides
different types of high-throughput screening procedures
the construction of biomimetic gold-and-silica nanoparticles, and
the construction of self-assembling quantum dots.
To use Biotin-dPEG®3-MAL, dissolve the product in a dry solvent and add it to an aqueous compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), care should be taken to use a minimal amount of organic solvent so as not to denature the biomolecule or cause salt precipitation.
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品牌介绍
Click Chemistry Tools(CCT)公司位于美国亚利桑那州斯科茨代尔(Scottsdale,AZ)。自公司成立以来,一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外,CCT还可以提供个性化的定制产品,已满足不同用户的需求。目前CCT的产品线主要包括:Azides(Biotin-Azides)试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。 Fluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ),自成立以来,一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外,Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。 Quanta Biodesign公司位于美国中部的俄亥俄州,成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合,生产出他们独有的专利产品dPEG Building Blocks and Linkers,被广泛用于细胞治疗和分子诊断等研究领域。另外,还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。 目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中
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