Lipoamido-dPEG®-TFP ester-100 mg

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Lipoamido-dPEG®4-TFP ester, product number QBD-10641, is a single molecular weight, discrete PEG (dPEG®) modifier of gold and silver surfaces, for example, on nanoparticles. The lipoic acid moiety on one end of the 24-atoms-long dPEG® spacer forms dative bonds with metals like gold and silver. The TFP ester reacts with free amines to form free amines, optimally in the pH range 7.5 - 8.0. The distance from the disulfide bond in the lipoic acid ring to the carbonyl carbon adjacent to the 2,3,5,6-tetrafluorophenol (TFP) ester, the distance is 24 atoms (28.0 Å). Applications for this product include creating self-assembled monolayers (SAMs) on metal surfaces and crosslinking and immobilizing peptides and proteins to the surfaces of metal nanoparticles. Lipoic acid, also known as alpha-lipoic acid, a-lipoic acid, and thioctic acid, consists of a five-membered ring containing a disulfide attached to a pentanoic acid group. As a naturally occurring antioxidant, lipoic acid functions as a cofactor in several enzyme systems. It is a bidentate thiol ligand that forms two stable dative bonds per lipoic acid group with gold, silver, and other metals. These dative bonds form with oxidized lipoic acid or reduced dihydrolipoic acid (DHLA). Because it forms two dative bonds on metal surfaces, lipoic acid is more stable than PEGylation reagents containing a single thiol. This increased stability prevents lipoic-acid-functionalized dPEG® products from being oxidized off the metal surface. The lipoic acid group forms dative bonds with metals with or without reduction to DHLA. If reduction to DHLA is preferred, Tris(2-carboxyethyl)phosphine (TCEP) will rapidly reduce lipoic acid to DHLA. TFP esters react more readily with amines and possess better hydrolytic stability than N-hydroxysuccinimide (NHS). The superiority of PEG-TFP esters over NHS esters is documented in the scientific literature. When using this product, it is typical to conjugate the carboxyl end of the dPEG® to an amine to form an amide bond before forming a dative bond between a metal surface and the lipoic acid moiety of Lipoamido-dPEG®4-TFP ester. The TFP ester reacts optimally at pH 7.5 – 8.0, but it will also react with amines at pH values above and below this range. Lipoamido-dPEG®4-TFP ester has limited solubility in water or aqueous buffer. We recommend using dry (over 3 Å molecular sieves) water-miscible solvents such as N, N-dimethylacetamide (DMAC) or acetonitrile to form a stock solution that can be added to aqueous buffer to carry out the reaction.
产品形式为淡黄色至琥珀色粘稠液体

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品牌介绍

Click Chemistry Tools（CCT）公司位于美国亚利桑那州斯科茨代尔（Scottsdale,AZ）。自公司成立以来，一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外，CCT还可以提供个性化的定制产品，已满足不同用户的需求。目前CCT的产品线主要包括：Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。 Fluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，自成立以来，一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外，Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。 Quanta Biodesign公司位于美国中部的俄亥俄州，成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合，生产出他们独有的专利产品dPEG Building Blocks and Linkers，被广泛用于细胞治疗和分子诊断等研究领域。另外，还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。 目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中

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