Bis-dPEG ®-NHS ester-1000 mg(1000mg)

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Bis-dPEG®17-NHS ester, product number QBD-10979, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a long (55 atoms, 64.4 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®17-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10979 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10979 is one in a line of similar products, Bis-dPEG®17-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules. NHS esters react with free amines such as the e-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®17-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.
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品牌介绍

Click Chemistry Tools（CCT）公司位于美国亚利桑那州斯科茨代尔（Scottsdale,AZ）。自公司成立以来，一致致力于不断开发和扩展一系列连接产品。他们利用特别新的研究成果研发生产出新颖的交联和标记试剂。产品被广泛用于药物研发、癌症治疗以及各类诊断技术等。此外，CCT还可以提供个性化的定制产品，已满足不同用户的需求。目前CCT的产品线主要包括：Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。 Fluoroprobes公司位于美国亚利桑那州斯科茨代尔(Scottsdale, AZ)，自成立以来，一直致力于不断开发一系列的连接(linker)产品。利用特别新的研究成果研发生产出各类新颖的交联和标记试剂。其产品被广泛用于药物研发与筛选、疾病治疗方法和诊断技术等领域。此外，Fluoroprobes还可以根据客户的个性化需求提供各类定制连接产品。代表产品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些标记试剂盒等。 Quanta Biodesign公司位于美国中部的俄亥俄州，成立于1997年。也是专注于生产研发各类标记产品的品牌之一。其研发的产品与dPEG技术相结合，生产出他们独有的专利产品dPEG Building Blocks and Linkers，被广泛用于细胞治疗和分子诊断等研究领域。另外，还可为广大科研工作者提供多种化学修饰试剂以及传统Liner等。 目前上述三个品牌都已被被Vectorlabs收购并整合到Vectorlabs产品中

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