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如何选择正确的同型对照(Isotype Controls)?

发布时间:2024-04-30 分享到:

如何选择正确的同型对照(Isotype Controls)?

选取的同型对照应与一抗的以下特征匹配:宿主种类、同种型、偶联形式

采用和一抗实验浓度相同的同型对照,以获得正确的结果分析。

什么是同型对照(Isotype Controls)

同型对照(Isotype Control)是指使用与一抗相同种属来源、相同亚型、相同剂量和相同的免疫球蛋白及亚型的免疫球蛋白。

为什么要使用同型对照(Isotype Controls)?

抗体在结构上都极其相似,只有一小部分可变区不同,决定其与抗原的特异性结合。抗体分子结构的剩余部分由保守区构成,在同型的抗体中其序列往往相同。

而抗体保守区是决定抗体作用机制的关键,它能参与调控并结合各类细胞上的Fc受体。所以同型对照(Isotype Control)可用于消除由于抗体非特异性与细胞结合而产生的假阳性干扰。同型对照也是阴性对照,其主要目的是确定一抗的结合是特异性的,而不是非特异性的Fc受体或与其他蛋白的相互作用。

 

同型对照

BioXcell作为体内研究抗体(in vivo antibodies)专家,可提供高纯度、低内毒素、无防腐剂的特点,具有非常高的性价比的体内实验同型对照抗体。

BioXcell同型对照抗体(Isotype Controls):

  • 可直接用于体内注射

  • 不含稳定剂、防腐剂

  • 超高纯度

  • 超低内毒素水平

  • 超高性价比


BioXCell热销产品:

货号产品名称中文名称同型对照稀释缓冲液
BE0091InVivoMAb  polyclonal Armenian hamster IgGInVivoMAb 多克隆亚美尼亚仓鼠 IgG
IP0070
BE0001-2InVivoMAb anti-human CD3InVivoMAb 抗人 CD3BE0085IP0070
BE0362InVivoMAb anti-human CEACAM 1/3/5/6/8InVivoMAb 抗人 CEACAM 1/3/5/6/8BE0083IP0070
BE0363InVivoMAb anti-human CEACAM7InVivoMAb 抗人 CEACAM7BE0083IP0070
BE0368InVivoMAb anti-human/ mouse/rat amyloid-betaInVivoMAb 抗人/小鼠/大鼠淀粉样蛋白-βBE0086IP0070
BE0378InVivoMAb anti-mouse CD16.2 (FcγRIV)InVivoMAb 抗小鼠 CD16.2 (FcγRIV)BE0091IP0070
BE0360InVivoMAb anti-mouse CD24InVivoMAb 抗小鼠 CD24BE0090IP0070
BE0012InVivoMAb anti-mouse CD25 (IL-2Rα)InVivoMAb 抗小鼠 CD25 (IL-2Rα)BE0088IP0070
BE0015-1InVivoMAb anti-mouse CD28InVivoMAb 抗小鼠 CD28BE0087IPT060
BE0015-5InVivoMAb anti-mouse CD28InVivoMAb 抗小鼠 CD28BE0091IP0070
BE0377InVivoMAb anti-mouse CD31 (PECAM-1)InVivoMAb 抗小鼠 CD31 (PECAM-1)BE0089IP0070
BE0001-1InVivoMAb anti-mouse CD3εInVivoMAb 抗小鼠 CD3εBE0091IP0070
BE0003-1InVivoMAb anti-mouse CD4InVivoMAb 抗小鼠 CD4BE0090IP0065
BE0119InVivoMAb anti-mouse CD4InVivoMAb 抗小鼠 CD4BE0090IP0070
BE0016-2InVivoMAb anti-mouse CD40InVivoMAb 抗小鼠 CD40BE0089IP0070
BE0017-1InVivoMAb anti-mouse CD40L (CD154)InVivoMAb 抗小鼠 CD40L (CD154)BE0091IP0070
BE0175InVivoMAb anti-mouse CD71 (TfR1)InVivoMAb 抗小鼠 CD71 (TfR1)BE0089IP0070
BE0024InVivoMAb anti-mouse CD80 (B7-1)InVivoMAb 抗小鼠 CD80 (B7-1)BE0091IP0080
BE0004-1InVivoMAb anti-mouse CD8αInVivoMAb 抗小鼠 CD8αBE0089IP0065
BE0061InVivoMAb anti-mouse CD8αInVivoMAb 抗小鼠 CD8αBE0090IP0070
BE0117InVivoMAb anti-mouse CD8αInVivoMAb 抗小鼠 CD8αBE0090IP0070
BE0213InVivoMAb anti-mouse CSF1R (CD115)InVivoMAb 抗小鼠 CSF1R (CD115)BE0089IP0070
BE0032InVivoMAb anti-mouse CTLA-4 (CD152)InVivoMAb 抗小鼠 CTLA-4 (CD152)BE0091IP0065
BE0131InVivoMAb anti-mouse CTLA-4 (CD152)InVivoMAb 抗小鼠 CTLA-4 (CD152)BE0087IP0070
BE0164InVivoMAb anti-mouse CTLA-4 (CD152)InVivoMAb 抗小鼠 CTLA-4 (CD152)BE0086IP0070
BE0241InVivoMAb anti-mouse IFNAR-1InVivoMAb 抗小鼠 IFNAR-1BE0083IP0070
BE0055InVivoMAb anti-mouse IFNγInVivoMAb 抗小鼠 IFNγBE0088IPT080
BE0371InVivoMAb anti-mouse IL-12 p35InVivoMAb 抗小鼠 IL-12 p35BE0089IP0070
BE0045InVivoMAb anti-mouse IL-4InVivoMAb 抗小鼠 IL-4BE0088IP0070
BE0075-1InVivoMAb anti-mouse Ly6GInVivoMAb 抗小鼠 Ly6GBE0089IP0070
BE0075InVivoMAb anti-mouse Ly6G/Ly6C (Gr-1)InVivoMAb 抗小鼠 Ly6G/Ly6C (Gr-1)BE0090IP0070
BE0036InVivoMAb anti-mouse NK1.1InVivoMAb 抗小鼠 NK1.1BE0085IP0070
BE0372InVivoMAb anti-mouse osteopontin (SPP1)InVivoMAb 抗小鼠骨桥蛋白 (SPP1)BE0366IP0070
BE0031InVivoMAb anti-mouse OX40 (CD134)InVivoMAb 抗小鼠 OX40 (CD134)BE0088IP0070
BE0033-2InVivoMAb anti-mouse PD-1 (CD279)InVivoMAb 抗小鼠 PD-1 (CD279)BE0091IP0065
BE0146InVivoMAb anti-mouse PD-1 (CD279)InVivoMAb 抗小鼠 PD-1 (CD279)BE0089IP0070
BE0273InVivoMAb anti-mouse PD-1 (CD279)InVivoMAb 抗小鼠 PD-1 (CD279)BE0089IP0070
BE0101InVivoMAb anti-mouse PD-L1 (B7-H1)InVivoMAb 抗小鼠 PD-L1 (B7-H1)BE0090IP0065
BE0353InVivoMAb anti-mouse/ human/rat/monkey ICOS (CD278)InVivoMAb 抗小鼠/人/大鼠/猴 ICOS (CD278)BE0091IP0070
BE0057InVivoMAb anti-mouse/ human/rat/monkey/ hamster/canine/bovine TGF-βInVivoMAb 抗小鼠/人/大鼠/猴/仓鼠/犬/牛 TGF-βBE0083IP0070
BE0083InVivoMAb mouse IgG1 isotype control, unknown specificityInVivoMAb 小鼠 IgG1 同种型对照,特异性未知
IP0065
BE0085InVivoMAb mouse IgG2a isotype control, unknown specificityInVivoMAb 小鼠 IgG2a 同种型对照,特异性未知
IP0070
BE0086InVivoMAb mouse IgG2b isotype control, unknown specificityInVivoMAb 小鼠 IgG2b 同种型对照,特异性未知
IP0070
BE0087InVivoMAb polyclonal Syrian hamster IgGInVivoMAb 多克隆叙利亚仓鼠 IgG
IP0070
BE0088InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidaseInVivoMAb 大鼠 IgG1 同种型对照,抗辣根过氧化物酶
IP0070
BE0089InVivoMAb rat IgG2a isotype control, anti-trinitrophenolInVivoMAb 大鼠 IgG2a 同种型对照,抗三硝基苯酚
IP0065
BE0090InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyaninInVivoMAb 大鼠 IgG2b 同种型对照,抗锁孔血蓝蛋白
IP0070
BE0099InVivoMAb recombinant CTLA-4-Ig (hum/hum)InVivoMAb 重组 CTLA-4-Ig(人/人-融合蛋白)BE0096IP0070
BE0098InVivoMAb recombinant Flt-3L-Ig (hum/hum)InVivoMAb 重组 Flt-3L-Ig(人/人-融合蛋白)BE0096IP0070
BP0307InVivoPlus anti-mouse CD16/CD32InVivoPlus 抗小鼠 CD16/CD32BP0090IP0080
BP0356InVivoPlus anti-mouse CD20InVivoPlus 抗小鼠 CD20BE0366IP0070
BP0012InVivoPlus anti-mouse CD25 (IL-2Rα)InVivoPlus 抗小鼠 CD25 (IL-2Rα)BP0088IP0070
BP0001-1InVivoPlus anti-mouse CD3εInVivoPlus 抗小鼠 CD3εBP0091IP0070
BP0003-1InVivoPlus anti-mouse CD4InVivoPlus 抗小鼠 CD4BP0090IP0065
BP0016-2InVivoPlus anti-mouse CD40InVivoPlus 抗小鼠 CD40BP0089IP0070
BP0017-1InVivoPlus anti-mouse CD40L (CD154)InVivoPlus 抗小鼠 CD40L (CD154)BP0091IP0070
BP0004-1InVivoPlus anti-mouse CD8αInVivoPlus 抗小鼠 CD8αBP0089IP0065
BP0061InVivoPlus anti-mouse CD8αInVivoPlus 抗小鼠 CD8αBP0090IP0070
BP0117InVivoPlus anti-mouse CD8αInVivoPlus 抗小鼠 CD8αBP0090IP0070
BP0213InVivoPlus anti-mouse CSF1R (CD115)InVivoPlus 抗小鼠 CSF1R (CD115)BP0089IP0070
BP0032InVivoPlus anti-mouse CTLA-4 (CD152)InVivoPlus 抗小鼠 CTLA-4 (CD152)BP0091IP0065
BP0131InVivoPlus anti-mouse CTLA-4 (CD152)InVivoPlus 抗小鼠 CTLA-4 (CD152)BP0087IP0070
BP0164InVivoPlus anti-mouse CTLA-4 (CD152)InVivoPlus 抗小鼠 CTLA-4 (CD152)BP0086IP0070
BP0241InVivoPlus anti-mouse IFNAR-1InVivoPlus 抗小鼠 IFNAR-1BP0083IP0070
BP0055InVivoPlus anti-mouse IFNγInVivoPlus 抗小鼠 IFNγBP0088IPT080
BP0045InVivoPlus anti-mouse IL-4InVivoPlus 抗小鼠 IL-4BP0088IP0070
BP0075-1InVivoPlus anti-mouse Ly6GInVivoPlus 抗小鼠 Ly6GBP0089IP0070
BP0075InVivoPlus anti-mouse Ly6G/Ly6C (Gr-1)InVivoPlus 抗小鼠 Ly6G/Ly6C (Gr-1)BP0090IP0070
BP0036InVivoPlus anti-mouse NK1.1InVivoPlus 抗小鼠 NK1.1BP0085IP0070
BP0031InVivoPlus anti-mouse OX40 (CD134)InVivoPlus 抗小鼠 OX40 (CD134)BP0088IP0070
BP0033-2InVivoPlus anti-mouse PD-1 (CD279)InVivoPlus 抗小鼠 PD-1 (CD279)BP0091IP0065
BP0146InVivoPlus anti-mouse PD-1 (CD279)InVivoPlus 抗小鼠 PD-1 (CD279)BP0089IP0070
BP0273InVivoPlus anti-mouse PD-1 (CD279)InVivoPlus 抗小鼠 PD-1 (CD279)BP0089IP0070
BP0101InVivoPlus anti-mouse PD-L1 (B7-H1)InVivoPlus 抗小鼠 PD-L1 (B7-H1)BP0090IP0065
BP0057InVivoPlus anti-mouse/human/rat/monkey /hamster/canine/bovine TGF-βInVivoPlus 抗小鼠/人/大鼠/猴/仓鼠/犬/牛 TGF-βBP0083IP0070
BP0089InVivoPlus rat IgG2a isotype control, anti-trinitrophenolInVivoPlus 大鼠 IgG2a 同种型对照,抗三硝基苯酚
IP0065
BP0099InVivoPlus recombinant CTLA-4-Ig (hum/hum)InVivoPlus 重组 CTLA-4-Ig(人/人-融合蛋白)
IP0070
IPT060InVivoPure pH 6.0T Dilution BufferInVivoPure pH 6.0T 稀释缓冲液

IP0065InVivoPure pH 6.5 Dilution BufferInVivoPure pH 6.5 稀释缓冲液

IP0070InVivoPure pH 7.0 Dilution BufferInVivoPure pH 7.0 稀释缓冲液

IPT080InVivoPure pH 8.0T Dilution BufferInVivoPure pH 8.0T 稀释缓冲液

SIM0008InVivoSIM anti-human CD20 (Rituximab Biosimilar)InVivoSIM 抗人 CD20(利妥昔单抗 生物类似药)BP0297IP0070
SIM0004InVivoSIM anti-human CTLA-4 (Ipilimumab Biosimilar)InVivoSIM 抗人 CTLA-4(伊匹单抗 生物类似药)BP0297IP0070
SIM0005InVivoSIM anti-human HER2 (Trastuzumab Biosimilar)InVivoSIM 抗人 HER2(曲妥珠单抗 生物类似药)BP0297IP0070
SIM0003InVivoSIM anti-human PD-1 (Nivolumab Biosimilar)InVivoSIM 抗人 PD-1(纳武单抗 生物类似药)CP147IP0070
SIM0010InVivoSIM anti-human PD-1 (Pembrolizumab Biosimilar)InVivoSIM 抗人 PD-1(帕博利珠单抗 生物类似药)CP147IP0070
SIM0009InVivoSIM anti-human PD-L1 (Atezolizumab Biosimilar)InVivoSIM 抗人 PD-L1(阿替利珠单抗 生物类似药)BP0297IP0070
SIM0001InVivoSIM anti-human TNFα (Adalimumab Biosimilar)InVivoSIM 抗人 TNFα(阿达木单抗 生物类似药)BP0297IP0070
SIM0006InVivoSIM anti-human TNFα (Infliximab Biosimilar)InVivoSIM 抗人 TNFα(英夫利昔单抗 生物类似药)BP0297IP0070
SIM0007InVivoSIM anti-human VEGF (Bevacizumab Biosimilar)InVivoSIM 抗人 VEGF(贝伐珠单抗 生物类似药)BP0297IP0070

*以上产品仅限科研用途,不得直接用于临床与医疗.   更多BioXCell产品手册


 

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