产品详情
绿色fS15靶向cADDis cAMP测定
Green fS15-targeted cADDis cAMP Assay
使用该标记的S15 cADDis cAMP传感器测量实时cAMP信号传导动力学。将fS 15标记的cADD添加到细胞中，并在第二天在荧光成像系统上测量稳健的信号。减少的绿色荧光指示cAMP增加。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括cAMP传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（B2 AR BacMam和10 mM异丙肾上腺素HCl溶液）。30 mL大小也是可用的。其他Montana分子工具，包括BacMam包装的GPCR，红色荧光核靶向或胞质cAMP传感器，或DAG或PIP 2的红色荧光传感器，可以很容易地与该测定共表达。
背景资料
Measure real-time cAMP signaling kinetics with this flag-tagged S15 cADDis cAMP sensor. Add fS15-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent nucleus-targeted or cytosolic cAMP sensors, or red fluorescent sensors for DAG or PIP2 can easily be co-expressed with this assay.
保存条件
4°C.
品牌介绍
Montana Molecular位于美国蒙大拿州波兹曼，开发了一系列基于BacMam系统的荧光生物传感器和报告系统，用于药物发现和活细胞研究，主要有GPCR检测，细胞应激和毒性相关检测工具。为我们的客户了解候选药物如何在长时间内影响细胞过程。在药物筛选过程的早期提供更多信息，以降低药物发现的风险和成本。这些产品帮助科学家观察和测量对维持健康和治疗疾病很重要的细胞过程。
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