终末补体复合物（TCC）ELISA 试剂盒评估人体免疫反应

《Longitudinal analysis reveals that delayed bystander CD8+ T cell activation and early immune pathology distinguish severe COVID-19 from mild disease》由 Laura Bergamaschi 等人撰写。文章通过对 COVID-19 患者的纵向分析，揭示了严重和轻度疾病在免疫反应上的差异，为深入理解 COVID-19 的免疫病理机制提供了重要依据。

研究团队通过对COVID-19患者的一系列样本进行免疫表型分析、RNA测序和血清细胞因子分析，分析了207名SARS-CoV-2感染者的免疫反应，这些感染者的疾病严重程度不同，研究时间跨度为症状出现后的12周。研究发现，轻症或无症状患者的早期免疫反应特征是强烈的旁观者CD8+ T细胞免疫反应，而不伴有系统性炎症。相比之下，住院患者的旁观者反应延迟，且在症状出现时就已显示出系统性炎症，这表明某些个体中免疫病理可能是不可避免的。

研究意义

这项研究的意义在于，它提供了对COVID-19免疫病理机制的深入理解，特别是在轻症与重症之间的差异。这些发现可能有助于开发新的治疗策略，特别是那些针对早期免疫反应和炎症途径的干预措施。通过了解补体系统在疾病中的作用，医生和研究人员可以更好地预测疾病进展，并为患者提供更个性化的治疗方案。HK328试剂盒作为测量补体系统激活状态的工具，在这一研究中发挥了重要作用，为理解COVID-19的免疫反应提供了重要数据。

终末补体复合物（TCC）ELISA 试剂盒，货号：HK328，在研究中的作用和价值

终末补体复合物（TCC），也称为sC5b-9，是评估人体免疫反应的重要生物标志物。该复合物在研究工作中至关重要，是机体先天防御机制的核心。HK328试剂盒在本研究中的作用是测量COVID-19患者血清中的TCC水平，这有助于研究人员评估补体系统在疾病中的作用。补体系统的激活与炎症反应密切相关，因此，通过HK328试剂盒测量TCC水平，研究人员可以：

1、评估炎症程度：TCC水平的升高可能表明补体系统被激活，从而反映了体内炎症反应的强度。

2、监测疾病进展：通过比较不同时间点的TCC水平，研究人员可以监测疾病的进展和患者的免疫状态变化。

3、理解免疫病理机制：TCC水平的变化可能揭示补体系统在COVID-19免疫病理中的具体作用，有助于开发新的治疗策略。

在实验中，HK328试剂盒提供了一种可靠和标准化的方法来测量TCC，这对于理解COVID-19的免疫反应至关重要。通过这种定量分析，研究人员能够更准确地评估补体系统在轻症与重症COVID-19中的不同作用，为疾病的诊断和治疗提供了重要信息。

产品详情：TCC ELISA, human, kit，货号：HK328

* 【相关推荐】 TCC单克隆抗体，推荐：TCC, Human, mAb aE11，货号HM2167

原理：

人类TCC ELISA是一种即用型固相酶联免疫吸附测定，基于夹心原理，工作时间为3?小时。两块板的高效格式配有十二个一次性8孔条带，允许自由选择检测批次的大小。样本和标准通过固相结合的特异性抗体进行捕获。生物素标记的追踪抗体将结合到捕获的TCC上。链霉亲和素-过氧化物酶复合物将结合到生物素标记的追踪抗体上。链霉亲和素-过氧化物酶复合物将与底物四甲基联苯胺（TMB）反应。通过加入草酸来终止酶反应。在450 nm处用分光光度计测量吸光度。通过绘制吸光度（线性）与相应的TCC标准浓度（对数）的关系获得标准曲线。样本的TCC浓度可以通过与标准同时进行的标准曲线来确定。

发表文章：

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