Rubisco | 557 kDa六聚体

背景资料

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

应用类型

Immunolocalization (IL), Western blot (WB)

免疫原

purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000), UniProt: P00875 and Q43832

宿主

兔

反应性

Anabaena sp. PCC 7120, 拟南芥, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechoc

保存条件

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

品牌介绍

Agrisera公司提供1000种用于植物和藻类细胞生物学研究的一抗，2000多种二抗。Agrisera公司提供的植物领域的抗体涵盖拟南芥、莱茵衣藻、蓝藻、松柏类、硅藻、大豆、大麦、蒺藜苜蓿、烟草、稻、菜豆、小立碗藓、杨树、豌豆、番茄、马铃薯、菠菜、小麦、玉米、葡萄等多个物种，此外还有水生生物类（藻类、鱼）抗体，细菌、昆虫、真菌抗体。针对植物蛋白质的保守氨基酸序列，Agirsera还独家开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。

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