mNeonGreen | Fluorescent Protein

背景资料

mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.

应用类型

Western blot (WB)

免疫原

KLH-conjugated peptide derived from synthetic peptide, UniProt: A0A1S4NYF2 from common lancelet Branchiostoma lanceolatum

宿主

兔

反应性

mNG tag in plant (拟南芥) and algal vectors (Chlamydomonas reinhardtii)

保存条件

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

品牌介绍

Agrisera公司提供1000种用于植物和藻类细胞生物学研究的一抗，2000多种二抗。Agrisera公司提供的植物领域的抗体涵盖拟南芥、莱茵衣藻、蓝藻、松柏类、硅藻、大豆、大麦、蒺藜苜蓿、烟草、稻、菜豆、小立碗藓、杨树、豌豆、番茄、马铃薯、菠菜、小麦、玉米、葡萄等多个物种，此外还有水生生物类（藻类、鱼）抗体，细菌、昆虫、真菌抗体。针对植物蛋白质的保守氨基酸序列，Agirsera还独家开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。

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